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Biotechnology Advances
Vol. 32, No. 8, 2014, Pages: 14291447

Recent insights into microbial catalases: Isolation, production and purification

Balwinder Singh Sooch, Baljinder Singh Kauldhar, Munish Puri

Enzyme Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, 147002, India.

Catalase, an oxidoreductase enzyme, works as a detoxification system inside living cells against reactive oxygen species formed as a by-product of different metabolic reactions. The enzyme is found in a wide range of aerobic and anaerobic organisms. Catalase has also been employed in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to its other applications in textile, paper, food and pharmaceutical industries. New applications for catalases are constantly emerging thanks to their high turnover rate, distinct evolutionary origin, relatively simple and well-defined reaction mechanisms. The following review provides comprehensive information on isolation, production and purification of catalases with different techniques from various microbial sources along with their types, structure, mechanism of action and applications.

Keywords: Hydrogen peroxide; Oxido-reductase; Haem-enzyme; Catalase peroxidase; Purification; Downstream processing; Protein structure.

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