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International Journal of Pure & Applied Bioscience
Vol. 4 (3), 2016, Pages: 130-135

Isolation and Purification of Cuticle Degrading Extra Cellular Proteases from Entomopathogenic Fungal Species of Beauveria bassiana

A.G. Vikhe, N.S. Dale, R.B. Umbarkar, G.B. Labade, A.R. Savant, and A.A. Walunj

Department of Agril. Entomology, College of Agriculture and Agril. Biotechnology, LONI-413736 Tal- Rahata, Dist-Ahmednagar, Maharashtra India.

Abstract

The entomotoxic protein of Beauveria bassiana (Bals.) (Ascomycota:Hypocreales) was precipitated with 90% saturated (NH4)2SO4 and the precipitate was eluted in Sephadex G-25 column. The protein content was higher (0.026 µg/ml) supported by UV spectroscopy absorbance. The vibration frequency in FT-IR appeared between the ranges of 1650– 1580cm-1 due to N-H bond formation for amines. Bioassays of Beauveria bassiana reveal that caused significantly higher insecticidal activity.Amylase, Protease and Lipase (digestive enzymes), Esterase, Glutathione S-Tranferase and Lactate dehydrogenase level/activity in insects. Hence, the Beauveria bassiana was subjected to sodium dedosyl sulphate–poly-acrylamide gel electrophoresis (SDS-PAGE)

Keywords: Beauveria bassiana, Protein Extraction, FT-IR, SDS-PAGE.

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