Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Bioresource Technology
Volume 307, 2020, 123249

Insight into the mechanisms of biochar addition on pollutant removal enhancement and nitrous oxide emission reduction in subsurface flow constructed wetlands: Microbial community structure, functional genes and enzyme activity

Yinkun Lianga,b, Qinghua Wanga, LeiHuanga,b, Maolin Liua, Ning Wanga,b, Yucheng Chena,b

Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment (Ministry of Education), College of Resource and Environment, Southwest University, Chongqing 400715, PR China.


A set of constructed wetlands (CWs) under different biochar addition ratios (0%, 10%, 20%, and 30%) was established to analyze the pollutant removal performance enhancement and nitrous oxide (N2O) emission reduction from various angles, including microbial community structure, functional genes and enzyme activity. Results revealed that the average removal efficiencies of ammonium (NH4+-N) and total nitrogen (TN) were improved by 2.6%–5.2% and 2.5%–7.0%. Meanwhile, N2O emissions were reduced by 56.0%–67.5% after biochar addition. Increased nitrogen removal efficiency and decreased N2O emissions resulted from the increase of biochar addition ratio. Biochar addition changed the microbial community diversity and similarity. The relative abundance of functional microorganisms such as NitrosomonasNitrospiraThauera and Pseudomonas, increased due to biochar addition, which promoted the nitrogen cycle and N2O emission reduction. High gene copy number and enzyme activity involved in nitrification and denitrification process were obtained in biochar CWs, moderating N2O emission.

Keywords: Biochar, Constructed wetland, Nitrous oxide, Microbial community, Enzyme.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution