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International Journal of Current Microbiology and Applied Sciences
Vol. 5 (3), 2016, Pages: 268-276

Extraction and Bio-chemical Characterization of Protease Enzyme from a Proteolytic bacteria Isolated from Dry Mixed Kitchen Waste

Diptendu Sarkar and Goutam Paul

New Horizon College of Engineering, Dept. of Biotechnology, Ring Rd, Kadubisanahalli, Bellandur Post, Near Marathalli, Bangalore-560103, India.

Abstract

The present research finding have shown that isolation and biochemical characterization of protease enzyme from proteolytic bacteria isolated from mixed vegetable kitchen waste. Three bacterium were isolated from kitchen waste and were screened for proteolytic enzyme production when grown on protein containing solid media, and designated them as DSP1, DSP2 and DSP3. DSP2 (found as Bacillus sp) was selected based on clearance zones and protease enzyme production was carried out in submerged fermentation. Enzyme production by DSP2 was higher at pH 6.0 and a temperature of 30°C using sucrose and ammonium sulphate as carbon source and nitrogen source, respectively. The maximal activity of DSP2 protease was at 30°C, pH 5.0 and was thermostable up to 60°C. Manganese sulphate had high effect on protease enzyme activity.

Keywords: Submerged Fermentation, Proteolytic Activity, Protease.

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