Cloning and characterization of a novel -amylase from fecal microbial metagenome
Xu B, Yang F, Xiong C, Li J, Tang X, Zhou J, Xie Z, Ding J, Yang Y, Huang Z
School of Life Science, Yunnan Normal University, Chenggong, Kunming, China.
To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of pygmy loris was constructed. The library was screened for amylolytic activity, and eight clones from the 50,000 recombinant clones showed amylolytic activity. The subcloning and sequence analysis of a positive clone led to the identification a novel gene (plA) coding for α-amylase. plA was expressed in Escherichia coli
BL21 (DE3) and the purified plA was enzymatic characterized. This study is the first to report the molecular and biochemical characterizations of a novel α-amylase from gastrointestinal metagenomic library.