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Biotechnology & Biotechnological Equipment
Vol. 32 (4), 2018, Pages: 1065–1074

AFM and MFM techniques for enzyme activity imaging and quantification

Michelle Arredondoa,b, Margarita Stoytchevab, Israel Morales-Reyesc and Nikola Batinac

College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.

Abstract

In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.

Keywords: Atomic force microscopy, magnetic force microscopy, trypsin activity, ferrogel.

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