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Geomicrobiology Journal
2016, Pages: 1521-0529

The succession of bacterial community structure in groundwater from a - 250-m gallery in the Horonobe Underground Research Laboratory

Kotaro Ise, Yoshito Sasaki, Yuki Amano, Teruki Iwatsuki, Isao Nanjo, Takahiro Asano & Hideki Yoshikawa

Japan Atomic Energy Agency, Nuclear Fuel Cycle Engineering Laboratories , 4 - 33 Muramatsu Tokai - Mura, Naka - gun, Ibaraki - ken 319 - 1194, Japan.

Abstract

We investigated the change in bacterial communit y structure after drilling boreholes, 09 - V250 - M02 and 09 - V250 - M03, in the 250 - m de ep research gallery of the Horonobe Underground Research Laboratory. In the 09 - V250 - M02 borehole , e - Proteobacteria w ere predominantly detected in the clone library analyses of the groundwater samples conducted immediately after drilling. All the e - Proteobacteria clones were closely related to Arcobacter spp., which are known to be sulfide - oxidiz ing c hemoautotrophic bacteria . A fter four years, the microbial structure drastically changed, and most detected operational taxonomic units were uncultured species such as candidate division OP9 and Chloroflexi relatives , which are frequently detected in deep - sea sediments. The results indicated that the microbial community structure was drastically affected by borehole drilling and was concomitant with oxidation perturbation. However, these disturbed microbial communities changed with in a few years to a microbial community compo sed of uncultivated speci es such as OP9 and Chloroflexi.

Keywords: deep subsurface microbiology, microbial ecology, high - level radioactive waste , geological disposal , redox potential , microbial biogeography.

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