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Ecological Engineering
Vol. 92, 2016, Pages: 595–597

Effects of root organic exudates on rhizosphere microbes and nutrient removal in the constructed wetlands

Zong-Jing Chen, Yun-Hui Tian, Yang Zhang, Ben-Ru Song, Hua-Chao Li, Zhang-He Chen

Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, and Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, PR China.

Abstract

Root organic exudates of four wetland plants were studied in wetland microcosms to test their effects on the density and diversity of the rhizosphere microbes, and on the nutrient removal.

An improved high-performance liquid chromatography (HPLC) was established to analyze the organic acids in the root exudates. The wetland plants had similar compositions, but different concentrations of root exudates. Root exudates might increase or decrease in the wastewater culture, varying with different exudates.

An innovative denaturing gradient gel electrophoresis (DGGE) was used to detect the microbe diversity and density. The microbe density and structure diversity of the microbe communities were different among the wetlands, and were higher in the rhizosphere than in the non-rhizosphere. The microbe density was generally positively correlated with organic exudates and negatively correlated with the pH of the culture solution, while the diversity showed reversed correlations.

Removal of chemical oxygen demand (CODCr), total phosphorus (TP), soluble phosphorus (SP), and ammonia nitrogen (NH4-N) had different correlations with the root exudates. Oxalate generally showed a positive correlation, while the pH generally presented a negative correlation. These correlations changed in the artificial wastewater culture. Nutrient removal was positively correlated with microbe density, while generally negatively correlated with diversity.

Root exudates contents were generally positively correlated with plant biomass, while negatively correlated with the pH. Microbe density was generally positively correlated, while the diversity was negatively correlated, with the biomass parameters. The results suggest that root exudates affect the rhizosphere microbe density and diversity, which affect the nutrient removal, and this might be one of the mechanisms of the root exudates affecting the constructed wetland nutrient removal process.

Keywords: Constructed wetland; Wetland plant; Root organic exudates; Rhizosphere microbes; Nutrient removal.

 
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