Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of the genus Herbaspirillum
Serrato RV, Sassaki GL, Cruz LM, Carlson RW, Muszynski A, Monteiro RA, Pedrosa FO, Souza EM, Iacomini M
Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
Bacteria from the genus Herbaspirillum are endophytes responsible for nitrogen fixation in gramineous plants of economic importance such as maize, sugarcane, sorghum, rice, and wheat. Some species are known to produce plant growth substances. In contrast, Herbaspirillum rubrisubalbicans strains are known to be mild plant pathogens. The molecular communication between the plant and the microbes might involve lipopolysaccharides present in the outer membrane of these gram-negative bacteria. Phenol-water extraction was used to obtain lipopolysaccharides from 7 strains of Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95, and M2) and H. rubrisubalbicans (M1 and M4). The electrophoretic profiles and chemical composition of the lipopolysaccharides obtained in the phenol and aqueous extracts were shown herein.
Keywords:Bacteria from the genus Herbaspirillum are endophytes,gramineous plants of economic,Herbaspirillum rubrisubalbicans strains.