Food Science and Technology Research
Vol. 20, No. 4, 2014, Pages: 899-904
Spoilage and Pathogenic Bacteria Associated with Spoilage Process of Sichuan Pickle during the Spontaneous Fermentation
Ke Li, Kai Lin, Zuogui Li, Qing Zhang, Feifei Song, Zhenming Che, Gong Chen, Wenliang Xiang
Key Laboratory of Food Biotechnology of Sichuan, College of Bioengineering, Xihua University.
Abstract
The spoilage and pathogenic bacteria in the sporadic microbial spoilage of Sichuan Pickle were investigated by 16S rRNA libraries. The pathogenic Vibrio penaeicida (39.19%), the spoilage and pathogenic Pseudomonas fluorescens (6.76%) were detected in initial spoilage stage. While, in middle spoilage stage they shifted to the pathogenic V. penaeicida (51.25%), the spoilage and pathogenic Pse. fluorescens (3.75%), the spoilage Pse. Chlororaphis (7.5%) and then to the pathogenic V. penaeicida (30.59%), Halomonas variabilis (10.59%) and Arcobacter marinus (5.88%), the spoilage Lactobacillus alimentariu (5.88%) in latter spoilage stage. The undesired Pseudoalteromonas nigrifaciens, Psychrobacter alimentarius, Marinomonas, Cobetia marina, Celerinatantimonas and V. litoralis were also detected in the spoilage process. When compared at species level, the community similarity coefficients of three samples were from 0.6122 to 0.5165 and ascended to 0.2359. These results are very useful to design the effective strategies to control or eliminate the microbial spoilage of Sichuan Pickle. aureus and Klebsiella pneumoniae were inhibited by probiotic.
Bacteriocin could potentially be used as food preservatives.
Environmental Information System (ENVIS) provides national and international
environmental information in the disciplines of pollution control,microorganisms,envis,
toxic chemicals, central and offshore ecology, environmentally sound and appropriate
technology, bio-degradation of wastes and environment management.
microorganims,envis,environment information system centre, microorganisms
and environment management, environment management, microorganisms, microbes
Environment Information System Centre - microorganims,envis,environment
management, microorganisms, microbes, pollution control, toxic chemicals,
central and offshore ecology, environmentally sound and appropriate technology,
bio-degradation of wastes.