Enzyme-free visualization of nucleic acids during HIV infection by octopus-like DNA
Jia Kong1, Yuefei Wang2, Wei Qi3, Rongxin Su3, Zhimin He1
State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China..
It is highly desirable to develop a facile approach for the detection of the human immunodeficiency virus (HIV). To address this need, an octopus-like DNA (OLD) is designed in a one-pot method by direct folding of nine short single strands of DNA (ssDNA), which can be used to capture all the conserved HIV-1 gene efficiently though the sticky arms. The branched OLD was applied for the enzyme-free detection of HIV-1 nucleic acid and the visualization of the virus during HIV infection. The total detection procedure can be finished within 2 h with high specificity, making the OLD system a valuable tool for the rapid detection of HIV virus and further biomedical application.
Keywords: Branched DNA, Enzyme-free, HIV detection.