Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
mn

Site Visitors

blog tracking


 
Carbohydrate Polymers
Vol.
93, No. 1, 2013; Pages: 154 - 162

Structural features and bioremediation activity of an exopolysaccharide produced by a strain of Enterobacter ludwigiiisolated in the Chernobyl exclusion zone

Corinne Pau-Roblot, Michelle Lequart-Pillon, Ludovic Apanga, Serge Pilard, Josiane Courtois, Nathalie Pawlicki-Jullian

BIOPI, Université de Picardie Jules Verne, 33, Rue St Leu, F-80039 Amiens, France.

Abstract

The bacterium Enterobacter ludwigii Ez-185-17, member of the family Enterobacteriaceae, was isolated from the root nodules of plants harvested in the nuclear power region of Chernobyl. Under batch culture conditions, the bacteria produce a high-molecular-mass exopolysaccharide (EPS). After purification, the structure of this EPS was determined using a combinatory approach including monosaccharide composition (GC-FID, HPAEC-PAD) and branching structure determination (GC–MS), as well as 1D/2D NMR (1H, 13C) and ESI-MS (HR, MS/MS) studies of oligosaccharides obtained from mild acid hydrolysis. The EPS was found to be a charged hexasaccharide with a repeating unit composed of d-galactose, d-glucose, l-fucose, d-glucuronic acid (2:1:2:1) and substituted with acyl and pyruvyl groups. The metal-binding properties of the exopolysaccharide were then investigated, and the results seem to indicate that the EPS decreased Cd sequestration in flax seeds.

Keywords: Enterobacter ludwigii; Exopolysaccharide; Structure; Bioremediation; Cadmium


 

 

 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution