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Bioresource Technology
Vol. 214, 2016, Pages: 234–241

Enhancing pesticide degradation using indigenous microorganisms isolated under high pesticide load in bioremediation systems with vermicomposts

Jean Manuel Castillo Diaz, Laura Delgado-Moreno, Rafael Núñez, Rogelio Nogales, Esperanza Romero

Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Department of Environmental Protection, C/Profesor Albareda, 1, 18008 Granada, Spain.


In biobed bioremediation systems (BBSs) with vermicomposts exposed to a high load of pesticides, 6 bacteria and 4 fungus strains were isolated, identified, and investigated to enhance the removal of pesticides. Three different mixtures of BBSs composed of vermicomposts made from greenhouse (GM), olive-mill (OM) and winery (WM) wastes were contaminated, inoculated, and incubated for one month (GMI, OMI and WMI). The inoculums maintenance was evaluated by DGGE and Q-PCR. Pesticides were monitored by HPLC-DAD. The highest bacterial and fungal abundance was observed in WMI and OMI respectively. In WMI, the consortia improved the removal of tebuconazole, metalaxyl, and oxyfluorfen by 1.6-, 3.8-, and 7.7-fold, respectively. The dissipation of oxyfluorfen was also accelerated in OMI, with less than 30% remaining after 30 d. One metabolite for metalaxyl and 4 for oxyfluorfen were identified by GC–MS. The isolates could be suitable to improve the efficiency of bioremediation systems.

Graphical abstract

Keywords: Agro-industrial vermicomposts; Bacteria–fungi consortium; Enhanced pesticide bioremediation; Q-PCR analysis; Pesticide metabolites.

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