petroleum-oil biodegradation efficiency of Bacillus subtilis
and Pseudomonas aeruginosa strains isolated from
a petroleum-oil contaminated soil from North-East India
Kishore Das, Ashis K. Mukherjee*
ONGC-Centre for Petroleum Biotechnology, Department of
Molecular Biology and Biotechnology, Tezpur University,
Tezpur 784 028, India.
The efficiency of Bacillus subtilis
DM-04 and Pseudomonas aeruginosa M and NM strains
isolated from a petroleum contaminated soil sample from North-East
India was compared for the biodegradation of crude petroleum-oil
hydrocarbons in soil and shake flask study. These bacterial
strains could utilize crude petroleum-oil hydrocarbons as
sole source of carbon and energy. Bioaugmentation of TPH contaminated
microcosm with P. aeruginosa M and NM consortia and
B. subtilis strain showed a significant reduction
of TPH levels in treated soil as compared to control soil
at the end of experiment (120 d). P. aeruginosa strains
were more efficient than B. subtilis strain in reducing
the TPH content from the medium. The plate count technique
indicated expressive growth and biosurfactant production by
exogenously seeded bacteria in crude petroleum-oil rich soil.
The results showed that B. subtilis DM-04 and P.
aeruginosa M and NM strains could be effective for in
aeruginosa, Total petroleum hydrocarbon (TPH).
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