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Bioresource Technology
Vol. 98, No: xx, 2007, Pages: : 1339–1345

Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India

Kishore Das, Ashis K. Mukherjee*

ONGC-Centre for Petroleum Biotechnology, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784 028, India.


The efficiency of Bacillus subtilis DM-04 and Pseudomonas aeruginosa M and NM strains isolated from a petroleum contaminated soil sample from North-East India was compared for the biodegradation of crude petroleum-oil hydrocarbons in soil and shake flask study. These bacterial strains could utilize crude petroleum-oil hydrocarbons as sole source of carbon and energy. Bioaugmentation of TPH contaminated microcosm with P. aeruginosa M and NM consortia and B. subtilis strain showed a significant reduction of TPH levels in treated soil as compared to control soil at the end of experiment (120 d). P. aeruginosa strains were more efficient than B. subtilis strain in reducing the TPH content from the medium. The plate count technique indicated expressive growth and biosurfactant production by exogenously seeded bacteria in crude petroleum-oil rich soil. The results showed that B. subtilis DM-04 and P. aeruginosa M and NM strains could be effective for in situ bioremediation.

Keywords:Bacillussubtilis,Biodegradation,Bioremediation,Biosurfactant,Polyaromatichydrocarbon(PAH),Pseudomonas aeruginosa, Total petroleum hydrocarbon (TPH).

Corresponding author: Tel +91 3712 267007/267008x5405; fax +91 3712 267006

E-mail: akm@tezu.ernet.in


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