A novel and
in situ technique for the quantitative detection of MTBE and
benzene degrading bacteria in contaminated matrices
J.P. Biggerstaffa,*, M. Le Puilb,
B.L. Weidowa, J. Leblanc-Gridleya,
E. Jenningsc, J. Busch-Harrisc, K.L.
Sublettec, D.C. Whitea, R.S. Alberteb
University of Tennessee, Knoxville, TN, United States.
A novel and in situ technique is presented
here as a better alternative to culture-dependent and PCR-based
techniques for the quantitative detection of predominant bacterial
species involved in the bioremediation of contaminants. It
allowed rapid, specific and in situ identification of Biosep®-immobilized
eubacteria from MTBE- and benzene-contaminated matrices.
Keywords: MTBE, Fuel oxygenate, Bioremediation,
FISH, Spectral imaging,methyl tertbutyl
Corresponding author: Tel +1 865 974 8021;
fax +1 865 974 8027