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Journal of Microbiological Methods
Vol.
78, No. 2, 2009; Pages: 242 - 244

Two novel transposon delivery vectors based on mariner transposon for random mutagenesis of Bacillus thuringiensis

Mingshun Li, Minglei Li, Wen Yin, Jin He, Ziniu Yu

State Key Laboratory of Agricultural Microbiology and National Engineering Research Center for Microbial Pesticides, Huazhong Agricultural University, Wuhan 430070, PR China.

Abstract

Two new mariner transposon delivery vector systems, pMarA333 and pMarB333, had been constructed to randomly mutagenize Bacillus thuringiensis in vivo. The results showed that both systems could randomly insert into the genome of B. thuringiensis YBT881 (CCAM 020673). These systems could have potential for further construction of mutant libraries of B. thuringiensis and other Bacillus strains.

Keywords: Bacillus thuringiensis; Mariner; Mutantagenesis; Transposon


 

 

 
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