Potential synergistic effect of Melia azedarach fruit extract and Beauveria bassiana in the control of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in cattle infestations
Lorena Alessandra Dias Sousa, Hélio Bernardes Pires Júnior, Sara Fernandes Soares, Pedro Henrique Ferri, Patricia Ribas, Eliane Martins Lima, John Furlong, Vânia Rita Elias Pinheiro Bittencourt, Wendell Marcelo de Souza Perinotto, Lígia Miranda Ferreira Borges
Laboratório de Parasitologia Veterinária, Universidade Federal de Goiás, Campus Samambaia (Campus II), CP 131 ZIP 74001-970, Goiânia, GO, Brazil.
The use of a concentrate emulsion of Melia azedarach green fruits and a suspension of the fungus Beauveria bassiana was evaluated in the control of Rhipicephalus microplus on artificially infested cattle. The evaluation was conducted following the protocol established by the Brazilian Agriculture Ministry. Five groups of 4 or 5 animals were allocated to one of the following treatments: emulsion concentrate of M. azedarach at 0.25% (T AZED 0.25%), emulsion concentrate of M. azedarach at 0.5% (T AZED 0.5%), B. bassiana at 2.4 × 108 conidia (T BASS), association of the concentrate of M. azedarach at 0.25% with B. bassiana at 2.4 × 108 conidia (T AZED 0.25% + BASS), and control (untreated). The association of the two compounds provided better results than any one isolated treatment, indicating compatibility or perhaps a synergy between M. azedarach and B. bassiana. This treatment resulted in fewer engorged females (129 ± 70) than in the control group (233 ± 82), showing high performance against all developmental stages of the tick. Results revealed an apparent synergistic effect of M. azedarach and B. bassiana in the control of R. microplus that should be further investigated.