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Journal of Asia-Pacific Entomology
Vol. 19 (Issue 3), 2016, Pages: 691–696

Identification and expression profiles of chitin deacetylase genes in the rice leaf folder, Cnaphalocrocis medinalis

Hai-Zhong Yu, Ming-Hui Liu, Xue-Yang Wang, Xin Yang, Wan-Ling Wang, Lei Geng, Dong Yu, Xue-Lan Liu, Gui-Ying Liu, Jia-Ping Xu

School of Life Sciences, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, People's Republic of China.

Abstract

Chitin deacetylase (CDA) is an insect chitin degradation enzyme that catalyzes the deacetylation of chitin to form chitosan. In this study, combination of rapid-amplification of cDNA ends (RACE) technology with Cnaphalocrocis medinalis transcriptome database analysis revealed the presence of at least five C. medinalis CDAs (CmCDAs), which were CmCDA1CmCDA2CmCDA4CmCDA5, and CmCDA6. The cDNA sequences ofCmCDA1CmCDA2, and CmCDA4 had whole open reading frame (ORF) for further analysis. Phylogenetic analysis indicated that insect CDAs could be categorized into five groups. CmCDAs structural domain analysis revealed that all three CDAs contained the chitin deacetylase-like catalytic domain. CmCDA1 and CmCDA2 belong to Group I because they both contain the chitin-binding peritrophin-A domain (ChBD), low-density lipoprotein receptor class A domain (LDLa), and chitin deacetylase-like catalytic domain.CmCDA4 only contains ChBD and chitin deacetylase-like catalytic domain thus belongs to Group III. Tissue and developmental stage expression analysis showed that the expression levels of CmCDA1CmCDA2, and CmCDA4 are significantly higher in the head than other tissues and also significantly higher in adults than in larvae. CmCDA5had significantly higher expression in integument than other tissues, suggesting potential roles in the process of degradation of chitin. In contrast, CmCDA5 showed relatively high expression in larvae. In conclusion, this study analyzed the cDNA sequences of threeCDA genes and determined their expression and molecular characteristics, which provided a new sequence resource and improved the development of bio-pesticides and the biological pest control and contributed to management of this important agricultural pest.

Graphical abstract

Keywords: Cnaphalocrocis medinalis; Chitin deacetylase (CDA); Developmental and tissue-specific expression.

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