2 1 2
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Food Chemistry
Vol. 226, 2017, Pages: 141–148

Cyclodextrin-based miniaturized solid phase extraction for biopesticides analysis in water and vegetable juices samples analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Li-Qing Peng, Li-Hong Ye, Jun Cao, Yan-xu Chang, Qin Lid, Mingrui An, Zhijing Tan, Jing-Jing Xu

College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.

Abstract

A cyclodextrin-based miniaturized solid-phase extraction was developed to extract biopesticides from water and vegetable juices. The analytes were detected by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. In the solid-phase extraction (SPE) procedure, the liquid sample solution is passed through a packed column filled with 40 mg of HP-β-CD, and then the target analytes are absorbed and finally eluted with methanol-acetic acid (90:10, v/v) into a collection tube. The limits of quantification ranged from 3.73 to 16.51 ng/mL for a water matrix, from 2.62 to 13.23 ng/mL for an orange juice matrix and from 1.76 to 10.35 ng/mL for a tomato juice matrix, respectively. The average recovery values were in the range of 88.3–95.9% for the spiked samples. The established methodology was successfully applied to analyze sanguinarine, berberine, rotenone and osthole in water, orange juice and tomato juice.

Keywords: Biopesticides; Cyclodextrin; Miniaturized solid-phase extraction; Ultra-high-performance liquid chromatography; Quadrupole time-of-flight mass spectrometry.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution