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Advances in Biochemical Engineering/Biotechnology
Vol. 138, 20
14; Page: 179 - 206

Cultivation of Marine Microorganisms in Single-Use Systems

Friederike Hillig, Maciej Pilarek, Stefan Junne, Peter Neubauer

Chair of Bioprocess Engineering, Technische Universität Berlin, Ackerstraße 76, ACK24, 13355, Berlin, Germany.


Marine cultures are an important source of novel substances and enzymes. As efforts to isolate strains from (deep) sea environments increase, the demand for methodology platforms to cultivate these organisms is also rising. Due to the high salt concentration and the shear sensitivity exhibited by some heterotrophic microalgae, single-use systems originally designed for the cultivation of mammalian cell lines can be a valuable alternative. Using the cultivation of the heterotrophic marine microalgae Crypthecodinium cohnii as an example, this chapter makes suggestions for experimental design, for improving process development by integrating parallel experiments, and for scaling-up and scaling-down methodologies. It describes how to identify suitable single-use systems and how to integrate a two-layer system with perfluordecalin to improve the gas transfer in deep-well plates. The process is also scaled up in several single-use systems. We also describe challenges in the process development to achieve sufficient oxygen transfer, monitoring, and control, and we discuss limitations such as corrosion, long-term stability, and leachables in single-use systems. Finally, we demonstrate a method for cheap, fast, and consistent process development for marine microorganisms.

Keywords: Crypthecodinium cohnii; Marine heterotrophic microalgae; Perfluorodecalin; Polyunsaturated fatty acid; Scale-up



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