Jhala YK, Shelat HN, Vyas RV and Panpatte DG
Department of Agricultural Microbiology, B. A. College of Agriculture, Anand Agricultural University, Anand 388110, Gujarat, India.
Endorhizospheric bacteria also known as endophytic bacteria that resides within living plant tissues without
substantially harming plants have found a large number of applications in today’s agriculture such as nutrient
cycling, tolerance to biotic and abiotic stress as well as promotion ofplant growth. In present study an attempt
has been made to test biodiversity of endophytic plant growth promoting bacteria from different plant species. As
nitrogen fixation is one of the prime mechanism of plant growth promoting activity exerted by microorganisms,
endophytic nitrogen fixing bacteria were isolated from from surface sterilized plant parts viz. root, stem and
leaves of species Cynodondactylon (Durva), Pothosscandens (Money plant), Ipomeabatata (Sweet potato),
Saccharumofficinarum (Sugarcane) cv. CO.LK-8001 and CO.-84135, Musa paradica (Banana) and Zea mays (maize)cv. GM-6 by using nitrogen free media selective for growth of nitrogen fixing bacteria. In all total 10
isolates were obtained from different plant species and parts and showed variation in their biochemical and
physiological characteristics. All the isolates were found to fix nitrogen and nitrogen fixation rates of all the
isolates ranged from 4.0 to 36.3 mg N fixed/gm of sugar consumed. Moreover, they also showed solubilizing tri
calcium phosphate in Pikovskaya’s broth.
Keywords: Endorhizospheric; Endophytic; Biochemical;