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Microbiological Research
Vol. 192, 2016, Pages: 30–36

Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles

Juan M. Palazzini, Christopher A. Dunlap, Michael J. Bowman, Sofía N. Chulze

Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, Río Cuarto, Córdoba, Argentina.

Abstract

Bacillus subtilis RC 218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). It was demonstrated to have antagonist activity against the plant pathogen under in vitroand greenhouse assays. The current study extends characterizing B. subtilis RC 218 with a field study and genome sequencing. The field study demonstrated that B. subtilisRC 218 could reduce disease severity and the associated mycotoxin (deoxynivalenol) accumulation, under field conditions. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the Bacillus velezensis clade. In addition, the draft genome allowed us to use bioinformatics to mine the genome for potential metabolites. The genome mining allowed us to identify 9 active secondary metabolites conserved by all B. velezensis strains and one additional secondary metabolite, the lantibiotic ericin, which is unique to this strain. This study represents the first confirmed production of ericin by a B. velezensis strain. The genome also allowed us to do a comparative genomics with its closest relatives and compare the secondary metabolite production of the publically available B. velezensisgenomes. The results showed that the diversity in secondary metabolites of strains in theB. velezensis clade is driven by strains making different antibacterials.

Graphical abstract

Keywords: Bacillus velezensis; FHB biocontrol; Genome sequencing; Secondary metabolite production; Lantibiotic ericin.

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