9 9 2 1 2
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Pesticide Biochemistry and Physiology
Vol. 140, 2017, Pages: 24-29

Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia

Lihua Zhao,Jiahong Dong, Zhonghui Hu,Shunlin Li, Xiaoxia Su, Jie Zhang,Yueyan Yin, Tao Xu, Zhongkai Zhang, Hairu Chen

College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.

Abstract

Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1β-methoxydiversifolin-3-0-methyl ether (Ses-5) were found to have in vivo curative activities of 62.86% and 60.27% respectively, at concentrations of 100 μg/mL, respectively. In contrast, the in vivo curative inhibition rate of control agent ningnanmycin was 52.48%. Indirect enzyme-linked immunosorbent assay (ID-ELISA) also verified Ses-2 and Ses-5 had higher inhibition activities than the control agent ningnanmycin. Additionally, qRT-PCR showed that both Ses-2 and Ses-5 can partly inhibit the expression of CP and RdRp, two genes that play key roles in TMV infection. When TMV started to systemically spread, Ses-2 inhibited CP expression while Ses-5 inhibited RdRp expression. These results suggest that the two bio-agents have anti-TMV activities and may be used as bio-pesticides to control the plant virus.

Graphical abstract

Keywords: Curative activities, Sesquiterpene, Half-leaf method, Bio-pesticides.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution