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Journal of Chemical & Pharmaceutical Research
Vol. 6, No: 3, 2014, Pages:
560-565

Characteristics and metabolic pathways of fluorene (FLU) degradation by strain Rhodococcus sp. USTB-C isolated from crude oil

Chan Yu; Jun Yao; Jingnan Jin

 

Abstract

A fluorene-degrading strain named USTB-C was isolated from crude oil of production well in Dagang oil field, southeast of Tianjin, northeast China. The 16S rDNA sequence of USTB-C showed 100% similarity with that of Rhodococcus erythropolis PR4, it was belonged to Rhodococcus. The strain USTB-C could remove 55.8% of fluorene with an initial concentration of 100 mg L-1 in 23 days without other substrates. It implied that the strain USTB-C showed the potential to degrading efficient of fluorene. Based on the intermediate metabolites analyzed by gas chromatography-mass spectrometry (GC-MS), we deduced the possible metabolic pathway of strain USTB-C for fluorene biodegradation. Fluorene was initially oxidized at C-9 positions finally resulting 9-fluorenone. Through hydrolysis reaction, 9-fluorene subject to form phthalic acid. Furthermore, phthalic acid was further degraded to 1 -hydroxybenzene. USTB-C evidently possesses efficient, high effective degrader and potential for further application on the enhanced bioremediation technologies for treating fluorene-contaminated soil.

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