Shape and size engineered cellulosic nanomaterials as broad spectrum anti-microbial compounds
Priyanka R. Sharma, Sunil Kamble, Dhiman Sarkar, Amitesh Anand, Anjani J. Varma
CSIR-National Chemical Laboratory, Polymer Science & Engineering Division, Dr. Homi Bhabha Road, Pune 411008, India.
Oxidized celluloses have been used for decades as antimicrobial wound gauzes and surgical cotton. We now report the successful synthesis of a next generation narrow size range (25–35 nm) spherical shaped nanoparticles of 2,3,6-tricarboxycellulose based on cellulose I structural features, for applications as new antimicrobial materials. This study adds to our previous study of 6-carboxycellulose. A wide range of bacteria such asEscherichia coli, Staphloccocus aureus, Bacillus subtilis and Mycobacterium tuberculosis (non-pathogenic as well as pathogenic strains) were affected by these polymers in in vitro studies. Activity against Mycobacteria were noted at high concentrations (MIC99 values 250–1000 μg/ml, as compared to anti-TB drug Isoniazid 0.3 μg/ml). However, the broad spectrum activity of oxidized celluloses and their nanoparticles against a wide range of bacteria, including Mycobacteria, show that these materials are promising new biocompatible and biodegradable drug delivery vehicles wherein they can play the dual role of being a drug encapsulant as well as a broad spectrum anti-microbial and anti-TB drug.
Keywords: Tricarboxycellulose; Cellulose; Spherical nanoparticles; Anti-microbial.