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Journal of Microbiological Methods
Vol. 117, 2015, Pages: 54–56

Protocols to test the activity of antimicrobial peptides against the honey bee pathogen Paenibacillus larvae

Jasmin C. Khilnani, Helen J. Wing

4505 South Maryland Parkway, Bldg WHI, M/S 4004, School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004, United States.


Paenibacillus larvae is the causal agent of the honey bee disease American Foulbrood. Two enhanced protocols that allow the activity of antimicrobial peptides to be tested against P. larvae are presented. Proof of principle experiments demonstrate that the honey bee antimicrobial peptide defensin 1 is active in both assays.

Keywords: Paenibacillus larvae; American Foulbrood disease; Antimicrobial compounds; Honey bee.

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