Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil
S.Usha Nandhini, S.Sudha, V.Anusha Jeslin, S.Manisha
Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Old Mamallapuram Road, Chennai 600119, Tamilnadu, India.
The main objective of the present study was to find the antimicrobial compounds from terrestrial Strptomyces sps. Streptomycetes strains were isolated from terrestrial soil and subjected to primary screened by cross streak method against some pathogenic Gram-positive and Gram-negative test bacteria and fungi. Streptomycetes of which one isolate showing broad spectrum of antimicrobial was characterized on the basis of their morphological and physiological properties. The antimicrobial substances were extracted from active isolates and inoculated in ISP2 broth were fermented for 7 days at 28°C by ethyl acetate extraction method. The active crude compounds were subjected to secondary screening by agar well diffusion method to confirm activity against same pathogenic bacteria and fungi. The crude compounds were purified by Thin layer chromatography (TLC). The bioactive region was detected on the TLC plate and calculated Rf value. Two bands were detected and each band tested for agar well diffusion method for antimicrobial activity. The characterization of the bioactive compounds was further determined by using UV–visible spectrophotometer, FT-IR and GC-MS techniques. These potent isolates from terrestrial soil could be an interesting source for pharmaceutical industries to explore antibacterial and antifungal compounds.
Keywords: Streptomyces, Antimicrobial compounds, Terrestrial soil.