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Journal of Microbiological Methods
Volume 162, 2019, Pages 50-61


Colorimetric microdilution assay: Validation of a standard method for determination of MIC, IC50%, and IC90% of antimicrobial compounds

P.Arulmozhia, S.Vijayakumara, P.K.Praseethab, S.Jayanthic

Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India.

Abstract

Capparis zeylanica Linn (Caparadaceae), a well-known traditional medicinal plant has been used prevalently in the Ayurvedic system of medicine. It has long been used in treating cholera, hemiplegia, pneumonia, helmintic and inflammatory activity. This study aims to investigate the antimicrobial activity of C. zeylanica leaf extracts against pathogenic microorganisms, with the interactions of potential compounds being predicted by a computational approach. Ethyl acetate leaf extracts of C. zeylanica were evaluated for antimicrobial activity using an agar well diffusion method against pathogenic microorganisms (Staphylococcus epidermidis, Enterococcus faecalis, Salmonella paratyphi, Shigella dysenteriae, Mycobacterium tuberculosis and Candida albicans). The ethyl acetate leaf extracts of the C. zeylanica were utilized for GC-MS analysis. Computational studies were performed to analyze the novel compound using Schrodinger software. The various concentrations of ethyl acetate leaf extract of C. zeylanica were checked against pathogenic microorganisms. Among them, Salmonella paratyphi shows the maximum inhibition. Molecular docking and ADME properties showed that (3E)-N-(3,4 Dichlorophenyl)-3-(Propionylhydrazono) butanamide, Heptadecanoic–Margaric acid and 5-(3-Fluorophenyl)-7-nitro-1,3-dihydro-2H-1,4-benzodiazepine-2-one had the highest fitness score and more specificity toward the microbial receptors.

Keywords: C. zeylanica, Antimicrobial activity, Well method, Molecular docking, ADME profile.

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